Journal: PLoS ONE

Article Title: Effects of Chronic Sleep Deprivation on the Extracellular Signal-Regulated Kinase Pathway in the Temporomandibular Joint of Rats

doi: 10.1371/journal.pone.0107544

Figure Lengend Snippet: Western blot technique was used to examine the possible mechanism by which pathological alterations occur in the TMJ. (A) Comparison of the p-ERK, ERK, MMP-1, MMP-3, and MMP-13 protein levels in the different groups as determined by Western blot (WB). (B) Mean relative protein levels of p-ERK, ERK, MMP-1, MMP-3, and MMP-13 in different groups (n = 10 per group). Bars represent the mean and SD of each group. CON, control; CSD, chronic sleep deprivation; d, day. ** P <0.01, * P <0.05.

Article Snippet: The following antibodies were used to detect proteins: rabbit anti-MMP-1 polyclonal antibody (1∶400 dilution; lot no. bs-4597R, Bioss, China), anti-MMP-3 (1∶500 dilution; lot no. BS1238, Bioworld, China), anti-MMP-13 polyclonal antibody (1∶500 dilution; lot no. BS-0575R, Bioss), anti-total ERK (1∶3,000 dilution; lot no. #1695, Cell Signaling Technology, USA), and anti-p-ERK (1∶1,000 dilution; lot no. #4370, Cell Signaling Technology).

Techniques: Western Blot

Journal: BMC Cancer

Article Title: Case report: long-term survival of an infant syndromic patient affected by atypical teratoid-rhabdoid tumor

doi: 10.1186/1471-2407-13-100

Figure Lengend Snippet: A , Schematic representation of the family tree of index case. Karyotype and SMARCB1/INI1 molecular evaluation ( E ) is reported (wt= wild type; Arg40X denotes the constitutional mutation found in index case). Present age and age of onset of clinical symptoms is given in years (y). B , Brain MRI of primary ATRT lesion. C , MRI of metastatic lesion. D , Hematoxylin eosin staining and E , SMARCB1/INI1 staining of ATRT tumor from index case, showing absence of SMARCB1/INI1 protein expression in cancer cells.

Article Snippet: Analysis of SMARCB1/INI1 copy number by real-time quantitative PCR was performed by means of relative quantification using standard curve method [ ], using TaqMan assays 4401631 (Applera) for RPPH1 endogenous control gene and Hs01497967_cn (Applera) for SMARCB1/INI1 .

Techniques: Mutagenesis, Staining, Expressing

Journal: BMC Cancer

Article Title: Case report: long-term survival of an infant syndromic patient affected by atypical teratoid-rhabdoid tumor

doi: 10.1186/1471-2407-13-100

Figure Lengend Snippet: A-C , Electropherogram of normal and tumor DNA-derived sequences showing the presence of heterozygous exon 2 c. 118C>T (Arg40X) in ( A ) patient’s constitutional DNA and the same homozygous mutation ( B ) in tumor samples. C , Parental sample, displaying normal DNA sequence. D-E , Fragment analysis ( D ) and histogram ( E ) of MLPA results for SMARCB1/INI1 gene dosage analysis in primary tumor tissue and in control samples. Red dots indicate peaks corresponding to SMARCB1/INI1 fragments.

Article Snippet: Analysis of SMARCB1/INI1 copy number by real-time quantitative PCR was performed by means of relative quantification using standard curve method [ ], using TaqMan assays 4401631 (Applera) for RPPH1 endogenous control gene and Hs01497967_cn (Applera) for SMARCB1/INI1 .

Techniques: Derivative Assay, Mutagenesis, Sequencing, Control